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Cells were pelleted by centrifugation (50 g for 5 minutes at 22 C) re-suspended in fresh media and the cell viability was determined by Trypan Blue exclusion

The dermis is highly vascularized with capillaries and contains a dense concentration of immune cells, making it the ideal route for diagnostic testing

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Wang L, Huang X, Kong G, Xu H, Li J, Hao D, Wang T, Han S, Han C, Sun Y, Liu X, Wang X

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