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NAD + is also involved in nucleotide and amino acid synthesis, such as the folate-dependent serine catabolism 347 , as well as genomic DNA replication, as reviewed above 118

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Then, 500 l of 2 HEPES-buffered saline (281 mM NaCl, 100 mM HEPES, 1.5 mM Na 2 HPO 4 , pH=7.12) was added drop wise and the solution was vortexed

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