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Secondary antibodies, Alexa 596 (#A-11012, Thermo Scientific, USA) and Alexa 488 (#A-21202, Thermo Scientific, USA) were used at 1:1000 dilution for double labeling for 30 min at RT

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Step 6 Inspect the Finished Solution Once the peptide has fully dissolved, inspect the solution under good lighting

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Vitamin D levels in children and adolescents with attention-deficit hyperactivity disorder (ADHD): a meta-analysis