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Further validation of the molecular mechanisms was performed using flow cytometry, Western blotting, and reverse transcription quantitative polymerase chain reaction (RT-qPCR)

No special timing requirements

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Under the supervision of a qualified medical professional, peptide therapy is generally very safe

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Cell Death Dis 7(12):e2511 Lee YM, Lee G, Oh TI et al (2016b) Inhibition of glutamine utilization sensitizes lung cancer cells to apigenin-induced apoptosis resulting from metabolic and oxidative stress