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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

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Anorectic thermogenic and anti-obesity activity of a selective orexin-1 receptor antagonist in ob/ob mice

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