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With no analytic standards available, an identification of unknown metabolites with HPLCUV/VIS technique is not possible

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An Orbitrap Fusion Lumos coupled to an Ultimate 3000 RSLC nano UHPLC equipped with a 100 m inner diameter 2 cm Acclaim PepMap Precolumn (Thermo Fisher Scientific) and a 75 m inner diameter 50 cm, 2 m particle Acclaim PepMap RSLC analytical column was used

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doi: 10.1038/nri.2017.1 93 MunckANray-Fejes-TthA