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Agreeably, we could only detect a non-significant tendency for reduced -arrestin 2 recruitment in our triple AA phospho-null mutant receptor despite significantly reduced internalisation in response to GLP-1 stimulation, with this effect more likely ascribed to the observed reduction in GRK2 recruitment, in agreement with a previous report highlighting GRK expression as required for GLP-1R internalisation (4)

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However, in bacterial GGTs, the donor binding site has been determined to be highly conserved, while the acceptor binding site is reported highly variable

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Further zooming into the impacts of GLP-1 on food consumption, a Morgan Stanley Research report provides interesting early insights on how GLP-1 users eating habits are changing (see figure below)