RPMI medium (Sigma-Aldrich 8758) or DMEM/F12 (Life Technologies 21331020;) supplemented with 22 mM l-glutamine (Life Technologies 25030024)
You can do this by alternating between different areas on the body such as the abdomen, thigh, or upper arm or simply choosing a different position at the same injection site
Here, FITC-conjugated agonists act as FRET acceptors when bound to GLP-1Rs labeled with lanthanide (terbium) SNAP-tag probes 42
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