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High levels of CaO in soft tissues may result in unregulated production of reactive oxygen species (ROS), provoking an inflammatory response and subsequently damaging the tissue 125

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Specifically, 2 mM FeSO 4 solution, 360 mg mL 1 safranine solution, 3% (wt%) H 2 O 2 solution, 0.1 M HCl (aq) and G/D-CuP were added to each well in a 96-well plate (n = 3)

Exogenous glutamate triggers stomatal closure via Ca 2+ -dependent protein kinases, while antagonists suppress this response (Yoshida et al