The process of freezing and then thawing a liquid peptide solution can cause ice crystals to form that physically damage the peptides structure, leading to significant degradation
Kalogjera L, Krezic I, Smoday IM, Vranes H, Zizek H, Yago H, Oroz K, Vukovic V, Kavelj I, Novosel L, Zubcic S, Barisic I, Beketic Oreskovic L, Strbe S, Sever M, Sjekavica I, Skrtic A, Boban Blagaic A, Seiwerth S, Sikiric P
Thymosins in multiple sclerosis and its experimental models: Moving from basic to clinical application
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Orphanet J Rare Dis 16 , 215 (2021)