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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

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6e and Supplementary Tables 4 and 512)

The medium was removed and 125 I-GLP-1 (7-36) NH 2 (60 pM) or 125 I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled peptide agonists were added for overnight incubation at 4 C