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Insoluble material was removed by centrifugation at 30,000 g for 30 min and the solubilised complex was immobilised by batch binding to M1 anti-FLAG affinity resin in the presence of 5 mM CaCl 2

In contrast, M2 macrophages generally favor iron export and exhibit anti-inflammatory functions

Visfatin induces the production of pro-inflammatory cytokines such as IL-1, IL-6, TNF-, and IL-8 in macrophages

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