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Some protocols recommend using higher concentrations (3% to 4%) specifically in these deep creases while using moderate concentrations elsewhere

blots were incubated with rabbit primary antibody anti-Id2 (c-20) (Santa Cruz Biotechnology, CA, USA), diluted 1750 in 1% (w/v) non-fat dry milk TBS-Tween (TTBS) overnight at 4C, or with tubulin (Santa Cruz Biotechnology, CA, USA), diluted 11000 in 1% (w/v) non-fat dry milk TTBS overnight at 4C with shaking

[3] Evans M, Lewis ED, Zakaria N, Pelipyagina T, Guthrie N

doi: 10.1111/j.1464-5491.2005.01743.x 129 JamaliZNazariMKhoramdelazadHHakimizadehEMahmoodiMKarimabadMNet al

doi: 10.3892/etm.2019.7579

When stored properly at -20C in a sealed container away from direct light and moisture, lyophilized GHK-Cu remains stable for 24-36 months from the date of manufacture