Additionally, the QCC members conducted training on the rational use of PPIs for key departments in our hospital, providing feedback on irrational use cases to improve the understanding of PPI indications among physicians in various departments
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Subsequently, the cells were stained with the Hoechst 33342 nuclear dye (Thermo Fisher Scientific, Inc.) at a 1:500 dilution in PBS at room temperature for 10 min, washed with PBS, and visualized using a BZ-X700 (Keyence Corporation)