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isolated by Dr Aya Farag from the University of Sheffield from a drainage site in Chesterfield, UK, and identified by 16S rRNA sequencing 111 ) were grown in BG11 medium (17.6 mM NaNO 3 , 0.23 mM K 2 HPO 4 , 0.3 mM MgSO 4 , 0.24 mM CaCl 2 , 0.031 mM citric acid, 0.021 mM ferric ammonium citrate, 0.0027 mM Na 2 EDTA, 0.19 mM Na 2 CO 3 , 1 ml trace metal mix per 1000 ml dH 2 O)
We also assessed the expression levels of key regulators involved in ferroptosis pathway including glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11), and transferrin receptor 1 (TFR-1), which is responsible for iron uptake
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Longterm storage is recommended at 20C, although shortterm stability may be maintained at 28C when protected from moisture and light
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This combination is particularly effective for individuals with genetic mutations like MTHFR that affect folate metabolism or methylation pathways FAQ How often do I need injections
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