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For 96-well plates, 10 L of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturers instructions) was added to wells containing 100 L of culture media and incubated for 30 min at 37 C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)

RESULTS Descriptive data of the children included in the study are represented in Table 1

The objective is weight management and not weight loss

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mTOR is considered to be an integrator of genetic and environmental cues, which confer predisposition to SLE 9,10,11

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