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have demonstrated that calorie restriction, but not metformin (in the diet 300 mg/kg/day), extended lifespan in male Fisher-344 rats, leading to the conclusion that metformin is not a calorie restriction mimetic (CRM) (78) (see Table 1 for a summary)

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Some studies push this even higher, suggesting 1.6 to 2.3 grams per kilogram of fat-free mass for optimal lean tissue preservation