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It may be a transient moiety because it can trigger protein aggregation and precipitation, which can obscure isolation of 2-O-His as an individual degradant (15)

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386390 The clear limitation of ischemic preconditioning is that it can only be deployed against a defined episode of acute hypoxia known to be imminent, such as occurs after artery clamping in transplant surgery, or the injection of radiocontrast medium