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Mice were acclimatized to the animal facility for 1 week before they received injections of MCF-7:2A human breast cancer cells: 2 10 7 cells were resuspended in 100 L PBS (Collaborative Biomedical Products, Bedford, MA, USA) and were bilaterally injected into the mammary fat pads of 20 ovariectomized mice

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When engineering Calcium Oxide Nanoparticles (CaO NPs) for biological applications, it is essential to consider their high reactivity and rapid conversion to Ca(OH) upon exposure to humidity and moisture