The PAR2-activating coagulation protease FXa ( F10 ), known to be expressed in various extrahepatic cell types, had low mRNA expression in the intestinal crypt cells at our sequencing depth, but a survey of recently published single-cell gene expression data showed that F10 was expressed in proximal enterocytes, whereas Tmprss2 and F2rl1 predominantly colocalized in the early enterocytes (36)
While the formal data is still forthcoming, the biochemical rationale is sound, compelling, and certainly worthy of rigorous scientific investigation
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The percentage of TUNEL-positive cells was calculated in Fiji from 5 regions per coverslip
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Discussion around coverage begins around 52:00 minutes.