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In all cases, including a therapeutic dose, it takes more than 48 hours for GSH to recover to its original steady-state

doi:10.1016/0022-2836(87)90191-4

Because inhomogeneous cellular distribution of reacted and unreacted probes may bias the ratiometric calculations, especially at the subcellular resolution, we opted for RT instead of RT-NH 2 to achieve high-quality quantitative results in living cells

J., Wallace, M., Saghatelian, A., & Metallo, C