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Cureus, 17(1), e78045

In contrast, a GSSR intermediate was detected and its decreased steady-state concentration at higher glutaredoxin concentrations was suggested to support monothiol mechanism (i) 60

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DSIP was first characterized by Marcel Monnier and colleagues at the University of Basel, Switzerland, arising from a series of cross-circulation experiments in which blood from a sleeping rabbit was transferred to an awake recipient, causing measurable changes in the recipient's EEG brainwave patterns consistent with delta-wave sleep induction

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The lysates were centrifuged (10.000 g , 30 min, 4 C) and the supernatants were loaded on equilibrated Ni-NTA agarose columns (Qiagen)