To exclude nonspecific amplification and/or the formation of primer dimers, control reactions were performed in the absence of target cDNA
To me it sounds like this could be the case with you
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Carnitine/acetylcarnitine also have potentially positive effects on hormonal and metabolic parameters in patients with reproductive dysfunction, such as polycystic ovary syndrome [86, 87], and these effects may involve a series of complex signal transduction pathways to maintain the homeostasis of the endocrine system [88]
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