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The Framingham Offspring Study

Without a preservative, though, it offers no protection against microbial growth once the vial has been accessed, which is why solutions reconstituted with sterile water carry a meaningfully shorter usable window than those prepared with bacteriostatic water

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Chapman LE, Darling AL, Brown JE (2016) Association between metformin and vitamin B12 deficiency in patients with type 2 diabetes: a systematic review and meta-analysis

( The rest of studies also had small sample size but some had longer follow-up period (up to 18 months)