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To determine if GLP-1R induces cAMP generation specifically at VAPB locations, we modified a fluorescence resonance energy transfer (FRET)-based system of biosensors targeted to endogenous proteins (FluoSTEPs) 25 , so that a FRET cAMP sensor would fully reconstitute only in the presence of the GFP 11 fragment fused to VAPB, resulting in functional biosensor assembly specifically at VAPB loci (Supplementary Fig

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Keywords: obesity, type 2 diabetes, bariatric surgery, weight loss, metabolic effects Background Chronic diseases lead to 7 out of 10 deaths every year in United States (US) and pose a major burden on health care accounting for 86% of national health care costs [1, 2]