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Protein samples were mixed with Laemmli sample buffer, boiled at 95C for 5 minutes, and separated on a 12% SDS-polyacrylamide gel
Ganglia were digested with collagenase A (1.5 mg ml 1 ) and trypsin (0.20 mg ml 1 ) for 1 h at 37 C and mechanically dissociated with fire-polished Pasteur pipettes
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Este preparado contiene 10 mg de DSIP liofilizado, suministrado en un vial de investigacin sellado