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Proteins were transferred at 4C onto a polyvinylidene difluoride membrane (Invitrogen) in NuPage Transfer Buffer (Invitrogen) with 20% (vol/vol) methanol

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This is because donation stimulates the production of new red blood cells, which havent been exposed to glucose for as long as the old ones

Journal reference: Jalleh RJ, Talley NJ, Horowitz M, Nauck MA (2026)

Future research is necessary to identify these areas and their potential role in the effects of GLP-1RAs