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Frequency: one subcutaneous injection each day at a consistent time, titrating up as tolerated over a 12-week cycle

Repeat with arms and buttocks in weeks 3 and 4

Published clinical trials used continuous daily administration for 12-24 weeks without scheduled breaks, establishing this as a viable approach with maintained safety throughout the treatment period

Larger individuals with more adipose tissue benefit from doses at the upper end of standard ranges

This established the mechanistic rationale for NNMT inhibition

The use of ultrasound-guided injections in the study ensured precise delivery of the treatment into the small rabbit knee joint, improving the accuracy and consistency of the intervention