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Cells on poly-D-lysine-coated coverslips were loaded with 2 M Fura-2 AM (Thermo Fisher Scientific, Waltham, MA) at 37 C for 40 min in DMEM

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287289 A study found that liraglutide therapy in mice decreased visceral fat, which is associated with metabolic diseases, and relatively increased subcutaneous fat, with lipogenesis being suppressed or enhanced, respectively