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Derivatization is a process by which a compound is chemically changed to a new compound that has properties suitable for a particular assay or particular analytical method and the agent that carries out this process is called a derivatizing agent

Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally

However, if you develop signs of infectionsuch as increasing redness, warmth, pus, or fevercontact your healthcare provider immediately