100 g NIP IgG1 variants were injected over the column and allowed to bind FcRn at pH 6.0 before being subjected to an increasingly more basic pH gradient (pH 6.0 to 8.8) over 110 min by mixing of two eluent buffers (20 mM MES sodium salt, 140 mM NaCl, pH 6.0 and 20 mM Tris/HCl, 140 NaCl, pH 8.8)
Administration Injection route: Delivered via subcutaneous injection, usually in the abdomen or thigh
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