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Recombinant proteins in the supernatant were purified using nickelSepharose resin (GE Healthcare) and initially washed with wash buffer B1 (50 mM Tris, 500 mM NaCl and 30 mM imidazole, pH 8.0) until no protein was detected in the eluent using the Bradford reagent

What to Expect Prior to your appointment, please make a list of any over-the-counter or prescription medications you're currently taking, as some medications can have an impact on weight

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Enpatoran in COVID-19 pneumonia: safety and efficacy results from a phase II randomized trial