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The RNAprotein complexes were captured on Dynabead protein A (Thermo Fisher, 10006D) at 4 C for 90 min, washed with NT2 buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM MgCl 2 and 0.05% NP-40) and then incubated in NT2 buffer containing DNase I (Qiagen, 79254) at 37 C for 10 min

The membranes were blocked with 5% nonfat dry milk in PBS supplemented with 0.1% (v/v) Tween 20 and incubated overnight at 4C with anti-CPT2, PPAR (Santa Cruz Biotechnology,United States), CPT1A, LCAD, Very long-chain acyl-CoA dehydrogenase (VLCAD), (Proteintech, United States), GAPDH (from Cell Signaling Technology, United States)

This lack of transparency is central to the product liability cases, raising questions about corporate responsibility, consumer safety, and the ethical obligations of pharmaceutical manufacturers

2014;9: e110235

The per-lot PDF is available as a paid add-on with your order, so your customers can verify the same lot independently when they need to

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