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Then, 1 g of total RNA was reverse transcribed via the HiScript III All-in-one RT SuperMix Perfect for qPCR Kit (Vazyme, China), and SYBR Green-based qRT PCR was performed using the Universal SYBR qPCR Master Mix Kit (Vazyme, China) on a QuantStudio 5 Real-Time PCR System (Thermofisher, USA) according to the manufacturers instructions

Naunyn Schmiedebergs Arch Pharmacol 397(1):599616

Consult with qualified healthcare providers for personalized treatment recommendations

Additionally, discussing any known allergies or previous reactions to injections with your healthcare provider can help tailor a safer treatment approach for you

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(Contribution to laboratory work) and E.T.Z