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Peptide 20, a GIPR/GLP-1R/GCGR triagonist (currently in phase 1 clinical trial) 17 with balanced potency at the three receptors, is evolved from a GLP-1R/GCGR dualagonist 18 through iterative sequence refinement and modification (Fig

3.7 Other PCT substances identification All results are summarized in Table 2

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TBST was used for washing then the diluted secondary antibody solution was added, and left for 1 h incubation