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PCR products were digested with restriction enzyme Cac8I at 37C overnight and the digested products were resolved by gel electrophoresis (6% polyacrylamide gel)

A novel strategy to activate cytoprotective genes in the injured brain

After incubation on ice for 1 h, the homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays

Single injection, two mechanisms

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Additionally, these individuals may also exhibit symptoms such as headaches, fatigue, and muscle aches