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TMT liquid chromatography-mass-spectrometry (LC-MS) analysis Samples were analyzed by liquid chromatography-tandem mass spectrometry using an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific) coupled to an UltiMate 3000 Series Rapid Separation LC system and autosampler (Thermo Scientific Dionex)

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Many patients experience a "stocking-and-glove" distribution, where the burning and tingling begin in the toes and fingers before slowly creeping up the limbs

Someone with significantly lower baseline growth hormone output may notice bigger shifts than someone whos already fairly optimized

This finding underscores the importance of individualized protein needs beyond muscle health, where 1.2-2g/kg/day is often recommended

These early changes tend to be subtle, such as reduced gut resilience or low-grade inflammation, long before obvious symptoms appear