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Cultures were immediately diluted ~1:20 in fresh LB (with IPTG, Mg 2+ , Ca 2+ , and 10 g/ml gentamicin) and 1 ml aliquots were gently mixed with phage lysates at an MOI-e of ~30100 (the actual MOI-e values were recorded the next day when cfu counts were obtained and are indicated in Supplementary Data 1)

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There are numerous examples of biased agonism at the GLP-1 receptor, including both natural and pharmacological orthosteric agonists