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N Engl J Med 383:15641576 Mohseni S, Tavakoli A, Ghazipoor H, Pouralimohamadi N, Zare R, Rampp T, Shayesteh M, Pasalar M (2025) Curcumin for the clinical treatment of inflammatory bowel diseases: a systematic review and meta-analysis of placebo-controlled randomized clinical trials

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Reconstitute with sterile bacteriostatic water or appropriate laboratory buffer Add solvent slowly along the vial wall to minimize foaming Gently swirl until the peptide is dissolved Store reconstituted solutions at 3646 F (28 C) Prepare aliquots where appropriate to reduce freezethaw cycles Careful preparation procedures help maintain peptide stability and solution consistency during laboratory experiments

Mol Genet Genomic Med 2019;7:e915

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KGN cells were transiently transfected using Lipofectamine 3000 (Invitrogen)