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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

(1996) Genotyping hepatitis C virus isolates from Spain, Brazil, China, and Macau by a simplified PCR method

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They also assessed biliary diseases, biliary cancer, cholecystectomy, cholecystitis, and cholelithiasis