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Animals were anaesthetized with ethyl ether after which a single intracolonic dose of 30 mg of TNBS dissolved in 0.25 ml of either 50% ethanol in saline (v/v) or saline, referred as TNBS+ethanol or TNBS+saline, respectively, was administered

Subsequently, by forming nano-spheres upon contact with biological fluids after oral administration, the in vivo pharmacokinetic parameters of the theobromine were significantly enhanced

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