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Immunoproteasome activity 20 g of liver samples from ibuprofen-treated and control animals were incubated with immunoproteasome buffer (50 mM Tris, 5 mM MgCl 2 , 20 mM potassium chloride (KCl), 1 mM DTT (freshly added), pH 7.5), and inhibitor or an equal volume of dimethyl sulfoxide (DMSO) for 20 min at room temperature

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J Biol Chem 273(18):1083510842 Rigobello MP, Folda A, Baldoin MC, Scutari G, Bindoli A (2005) Effect of auranofin on the mitochondrial generation of hydrogen peroxide

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