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Briefly, each component was pooled in volume of 20 l with final concentration as follows before injection: 0.25 ng/l HiFi Cas-9 (IDT, #1081061), 30 M tracrRNA (IDT, #1072534), 15 M lag-1 specific crRNA(atacagtaatcccgcgagagNGG) (IDT, Alt-R TM ), 0.02 M lag-1 specific single strand DNA repair templates (3xHA sequences are underlined) (cctacaaatcattggaacgacatggaccgtgcagaattgtgtccaattacTACCCTTACGACGTGCCAGATTACGCTTACCCCTACGACGTACCAGACTACGCCTACCCATACGACGTCCCAGACTACGCTTAGattAAactctcgcgggattactgtatctttatattgtctcctaatttctcccaattcgt) (IDT), 15 M pha-1 crRNA and 0.02 M pha-1(e2123) specific repair template [67]

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