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Indian J Exp Biol Manning MC et al

Freezing irreversibly damages the peptide structure, reducing or eliminating efficacy

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This temporal pattern is consistent with the well-described phenomenon of GI adaptation to GLP-1 receptor activation, in which chronic receptor stimulation leads to desensitization of the vagal afferent pathways that mediate nausea and the enteric nervous system circuits that regulate gastric emptying and motility

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