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The tubes were kept in a boiling water bath for 30 min, cooled, and read at 535 nm

The cells were then incubated with continuous shaking at 45 C for 30 min, before being plated on HC medium supplemented with 2% (w/v) glucose as a carbon source and lacking histidine to ensure plasmid retention

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Well-defined nonapeptide sequence commonly used for assay standardization and comparative research