The study was reviewed and approved by the IRB of Samsung Medical Center (IRB approval number: 2021-06-064) and all methods were performed according to the IRB committees regulations
The PCR initial activation step was performed at 95C for 15 min to activate the polymerase, followed by 40 three-step cycles of 15 min at 94C, 30 min at 55C and 30 min at 72C
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Shan, F., Shao, Z., Jiang, S
Investigating therapeutic tolerance (CDK1) : Not all promising targets prove to be effective, and understanding the mechanisms of therapeutic failure is as important as identifying successes