Further, it suggests that the inflammatory response is also independent of plaque burden
doi:10.1186/s13023-022-02179-y
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Step 3: Verify Concentration and Label the Vial with Reconstitution Date After reconstitution, verify your concentration matches the mix 5-Amino-1MQ calculator result
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Due to the abundance of endogenous biotinylated proteins, especially carboxylases that use biotin as a cofactor (such as POD-2/acetyl-CoA carboxylase, PCCA-1/propionyl-CoA carboxylase, PYC-1/pyruvate carboxylase, and MCCC-1/methylcrotonyl-CoA carboxylase), which can reduce TurboID sensitivity, in 2022, the authors added a C-terminal His10 tag to these genes and subsequently removed them from the worm lysate using immobilized metal affinity chromatography
