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In brief, each DNA sample (10 ng) was assessed for the telomere and the single-copy gene (hemoglobin-b, HBB ) in triplicate within the same 384-well plate, amplified by using Platinum SYBR Green and 0.5 M of each primer in 10 l total reaction volume

[DOI] [PMC free article] [PubMed] [Google Scholar] 8.Choi H.K., Atkinson K., Karlson E.W., Willett W., Curhan G

147 Leptin binds to the leptin receptor (LepRb) and activates JAK2/STAT3 pathway to decrease body weight and normalizes blood glucose concentration, meanwhile, JAK2 stimulates the phosphorylation of insulin receptor substrate (IRS1/2), then activates PI3K/Akt pathway and directly affect insulin action

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doi: 10.1055/s-0035-1569330, PMID: [DOI] [PubMed] [Google Scholar] 147