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After removing the medium, SAC solutions (1.25, 2.5, 5, 10, and 20 mM) prepared in complete medium were added to respective wells

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Fermented foods containing probiotics might support GLP-1 production by influencing gut microbiota, though evidence remains preliminary

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Immunoprecipitation For immunoprecipitation assays, NIH-3T3 cell or tissue protein, respectively, was extracted with a non-denaturing cell or tissue extraction buffer (Invitrogen/ThermoFisher, Carlsbad, CA)

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