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Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco), 1 mM sodium pyruvate (Gibco) and 100 units/mL penicillin and 100 g/mL streptomycin at 37 C in 5% CO 2

Nature (2012) 483:3504

During reconstitution Step 4: Calculate your water volume

If you need to mix it after adding water, swirl gently

The radioprotection studies of DHS were carried out in a mice model wherein intraperitoneal administration of DHS (2 mg/kg body weight for five consecutive days before irradiation and three times per week during the post-irradiation period) showed significant improvement in the 30-day survival against the lethal dose (8 Gy, -rays) of WBI (Table 2) [64]

The anti-inflammatory marker CD68 was detected using a primary anti-CD68 antibody (Lab Vision, Neomarkers, USA) for 90 min